Description
DNA Oligonucleotide
| Short, single-stranded DNA molecules designed to specifically bind complementary DNA sequences. |
| Essential tools in molecular biology for amplification, detection, and modification of genetic material. |
| Includes standard DNA primers, LNA-modified primers, phosphorothioate-modified primers, and primers with various terminal modifications. |
| Production & Quality Assurance |
| Synthesized using high-purity reagents under contamination-free conditions. |
| Sequence fidelity and concentration validated by LC-MS and spectrophotometry. |
| Key Features |
| Custom synthesis 20–150 bases |
| Multiple purification options |
| Minimal secondary structure |
| High sequence fidelity |
| Lyophilized for long-term stability |
| Purification Options |
| Desalt: routine PCR and standard applications |
| OPC/RPC: high-purity applications |
| HPLC: sensitive downstream application |
| Preparation |
| To prepare lyophilized oligonucleotides as a 100 μM stock product, they should be reconstituted with nuclease-free water or TE buffer at the volume specified during quality control. |
| Do not vortex; mix by pipetting. The required volume is stated on the product label and in the user guide. |
| Required volume stated on product label and user guide |
| Shelf Life |
| Lyophilized probes stable 1 year at +4°C; |
| after dissolution in nuclease-free water or TE buffer, stable up to 6 months at -20°C. |
| Delivery |
| Shipped within 1–3 business days in lyophilized, vacuum-sealed, moisture-protected tubes. |
| The accompanying guide includes: Sequence information, Scale and Purification method, Sequence Length, Tm, GC, MW, nmol, A260, OD, µl required for 100 µM stock |
| Common Applications |
| PCR & qPCR |
| DNA Cloning & Sequencing |
| Genotyping & SNP Analysis |
| Mutation Detection |
| Primer-based Hybridization |
