Custom Designs for Reliable Amplification
We provide fully customized primer and probe designs tailored to your experimental needs. Each design is optimized for high specificity, balanced GC content, and consistent amplification efficiency, ensuring accurate and reproducible results. By integrating advanced bioinformatics tools with molecular biology expertise, we minimize the risk of non-specific binding and primer–dimer formation.
Why Choose a Designed Primer/Probe?
Our designs offer several key advantages:
- Target-specific optimization for accurate amplification
- Minimized secondary structure and primer–dimer risks
- Compatibility with singleplex and multiplex assays
- Multipurpose usage in PCR, qPCR, genotyping, sequencing, mutation detection, and site-directed mutagenesis
- If the primers, probes, or positive controls designed for your qPCR assay are either created by us or provided by you, we can optionally perform qPCR-based quality control upon request.
Applications by Type:
PCR/qPCR: Reliable amplification and quantification of target sequences
Genotyping & SNP analysis: Accurate detection of allelic variants
Mutation detection & sequencing: High-fidelity primers/probes for variant identification
Cloning & site-directed mutagenesis: Optimized primers for precise modifications
Ordering Considerations
To create a primer/probe design perfectly suited for your study, please provide the following information:
Target organism: Specify the organism to ensure sequence specificity
Target region (gene): If applicable, define the region of interest
Intended application: PCR, qPCR, genotyping, sequencing, mutation analysis, etc.
Oligonucleotide type: Forward primer, reverse primer, probe, or positive control (multiple selections allowed)
Singleplex or multiplex setup: Determines design constraints for multiplexing
Target channel information: If multiplexing with fluorescent probes
Providing this information allows us to tailor the design for optimal binding, amplification efficiency, and overall assay performance.
Delivery Stage
- Delivery time depends on the complexity of the design and selected quality-control options.
- Each design is accompanied by a detailed guide containing sequence information, purification method, and absorbance data (A260, OD, nmol, etc.).
