Custom Oligonucleotides for Reliable Performance
We synthesize high-quality DNA oligonucleotides (primers) tailored for a wide spectrum of molecular biology applications. Our primers deliver precise target recognition, efficient hybridization, and reliable amplification performance across PCR, qPCR, genotyping, sequencing, and mutagenesis assays. Our portfolio includes standard DNA primers, LNA-modified primers, phosphorothioate-modified primers, and primers with various terminal modifications.
Note: Terminally modified primers (biotin, thiol, digoxigenin, phosphorylation, Tamra, azide, alkyne, and internal spacer) are available through our TaqMan® Probe Order Page.
Production & Quality Assurance
Each primer is synthesized using high-purity reagents under stringent contamination-free conditions, ensuring optimal coupling efficiency and sequence fidelity. All oligonucleotides undergo rigorous quality control, including LC-MS and spectrophotometric analysis to assess sequence accuracy, purity, and concentration.
Lyophilized primers maintain stability for up to 1 year at +4°C. Once reconstituted in nuclease-free water or TE buffer, primers remain stable for up to 6 months at -20°C, depending on buffer composition and pH.
Purification Guidance
We offer multiple purification options to suit diverse experimental needs. Choose the method that best matches your application to ensure optimal performance and reliability.
Desalt: Suitable for routine PCR and standard applications
OPC/RPC: Optimal for applications requiring higher purity and minimal byproducts
HPLC: Recommended for sensitive downstream applications such as cloning, sequencing, or mutational analysis
Delivery Stage
- Orders shipped within 1–3 business days
- Primers are delivered lyophilized in vacuum-sealed, moisture-protected tube
- The product is accompanied by a detailed guide containing sequence information, purification method, and absorbance data (A260, OD, nmol, etc.).
Key Features
| Custom synthesis from 20 to 150 bases |
| Multiple purification options |
| Minimal secondary structure formation |
| High sequence fidelity and consistent amplification efficiency |
| Contamination-free and nuclease-free production environment |
| Lyophilized format for long-term stability |
Common Applications
| PCR and qPCR amplification |
| DNA cloning and sequencing primers |
| Genotyping and SNP analysis |
| Mutation detection assays |
| Primer-based hybridization assays |
