Oligonucleotide Order:
- Select Oligonucleotide from the “Products” menu.
- Choose the currency.
- Enter the sequence name.
- Type the nucleotide sequence (A, T, G, C, etc.).
- Select the purification method.
- Choose the synthesis scale.
- Price will be calculated automatically.
- Click “Add to Cart.”

TaqMan® Probe Order:
- Select TaqMan® Probe from the “Products” menu.
- Choose the currency.
- Enter the sequence name.
- Type the nucleotide sequence (A, T, G, C, etc.).
- Select the appropriate modification.
- Choose the synthesis scale.
- Price will be calculated automatically.
- Click “Add to Cart.”

qPCR Kit Order:
- Select qPCR Kits from the “Services” menu.
- Fill in required contact and company details.
- Select kit type and reaction number.
- Click “Submit.”
- Our team will contact you.

Other Services:
- Select the service from the “Services” menu.
- Fill out the request form.
- Click “Submit.”
- Our team will contact you.

- Click the Custom Order Form button.
- Download the Excel order form and read the instructions carefully.
- Add product details with correct specifications.
- Upload the completed file via the Special Order Form page.
- Fill in required information and click “Send Form.”

To prepare lyophilized oligonucleotides and TaqMan® Probes as a 100 μM stock product, they should be reconstituted with nuclease-free water or TE buffer at the volume specified during quality control.
Do not vortex; mix by pipetting. The required volume is stated on the product label and in the user guide.

Reconstituted oligonucleotides and TaqMan probes should be stored at -20 °C. Lyophilized (unreconstituted)
products can be kept at +4 °C. To ensure long-term stability, minimize freeze–thaw cycles.

The shelf life of oligonucleotides is determined by a combination of physical, chemical, and biological factors:
Storage conditions: Temperature, physical form (lyophilized or in solution), and duration of storage directly
influence stability. Lyophilization allows extended storage, even at room temperature.
Purity level: Oligonucleotides purified by HPLC are free from salts and by-products, resulting in longer shelf life.
Chemical and environmental sensitivity: Fluorescent dye–labeled probes are light-sensitive; exposure to UV
or intense light can cause structural degradation. Protective buffer components (e.g., EDTA) help inhibit nuclease
activity and improve stability.
Contamination risk: Improper handling can introduce microorganisms or nucleic acids, leading to degradation.
Freeze-thaw sensitivity: Single-stranded oligonucleotides are susceptible to structural damage from repeated
freezing and thawing cycles. Therefore, it is recommended to prepare master stocks once and store aliquots to
minimize freeze-thaw cycles.
By considering all these factors and ensuring proper storage and handling, oligonucleotides can maintain optimal
performance for extended periods.

Our standard production covers oligonucleotides ranging from 20 to 150 bases in length. Oligonucleotides shorter than 20 bases can also be synthesized, but working with such short sequences may reduce the reliability and quality of results. For longer sequences, customized production planning is available. Please contact our team for detailed information and specific requirements.

Our products are ready-to-use for common molecular biology applications such as PCR, qPCR, DNA cloning,
and Sanger sequencing. With our custom-designed oligonucleotides and probes, we provide application-specific optimization for research, diagnostics, and advanced molecular analysis.

Desalt – Sufficient for basic analyses and low-sensitivity applications.
RPC – Suitable for routine work requiring moderate purity.
OPC – Recommended for qPCR and sensitive quantitative assays.
HPLC – Ideal for high-precision, low-error-tolerance experiments and modified oligos

To select the appropriate fluorescent dye, consider the excitation light source, optical filters, and detector
sensitivity of your qPCR instrument. Important parameters include the dyes’ emission and absorption spectra,
Stokes shift, photostability, and quantum yield. Please refer to the “Fluorescent Dyes” tab in the TaqMan Probe
section of our website for detailed information on available dyes. For dyes not listed, contact us.

Standard orders are typically ready within 1–3 business days. However, selected modifications, purification
methods, or changes in production processes may affect delivery time. Please check the Announcements section
on our website for the latest updates.

If the product has passed quality control, performance issues are often related to design, instrument settings,
or experimental conditions. Check:
- Primer/probe design and proper Tm
- Instrument calibration
- Vortex use during reconstitution (dye efficiency)
- Number of freeze–thaw cycles
- Whether repeat tests were performed
If the issue persists, share your workflow with us so we can troubleshoot together.